{"ok":true,"agent":{"key":"biotech-lab-protocol-sop-en","name":"Lab Protocol / Standard Operating Procedure","category":"biotech-lifesciences","locale":"en","price_usd":35,"avg_turnaround_minutes":5,"revisions_included":2,"model":"anthropic/claude-sonnet-4-6","output_format":"markdown","input_fields":[{"id":"protocol_title","label":"Protocol / SOP title","type":"text","required":true,"placeholder":"e.g. Western Blot for Phospho-STAT3, Cell Viability Assay (MTT), RNA Extraction from Tissue Samples"},{"id":"purpose","label":"Purpose and scope of this protocol","type":"textarea","required":true,"placeholder":"e.g. To reliably detect phosphorylated STAT3 (Y705) in cancer cell line lysates using standard Western blot. Applies to all mammalian cell lines maintained in our lab."},{"id":"materials","label":"Key materials, reagents, equipment","type":"textarea","required":true,"placeholder":"e.g. Cell lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 1% NP40 + protease inhibitors), BCA protein assay kit, primary Ab: anti-p-STAT3 (Cell Signaling #9145), secondary Ab: anti-rabbit HRP, SDS-PAGE gel (10%), PVDF membrane, ECL reagent, chemiluminescence imager"},{"id":"procedure_steps","label":"Key procedure steps or outline","type":"textarea","required":true,"placeholder":"e.g. 1. Lyse cells on ice 20 min. 2. BCA assay, normalize to 30 µg protein. 3. Run SDS-PAGE. 4. Transfer to PVDF. 5. Block 5% milk/TBST 1hr RT. 6. Primary Ab overnight 4°C. 7. Wash, secondary, ECL..."},{"id":"safety_notes","label":"Safety / hazard notes","type":"textarea","required":false,"placeholder":"e.g. SDS is a skin/eye irritant — use nitrile gloves and lab coat. Acrylamide (if casting gels) is a neurotoxin — pre-cast gels recommended."},{"id":"expected_result","label":"Expected result / acceptance criteria","type":"text","required":false,"placeholder":"e.g. Distinct band at ~92 kDa for p-STAT3; loading control (β-actin) band at ~42 kDa uniform across lanes"}],"guardrails":["no_fabricated_statistics","no_false_claims"]}}